Illumina UD Indexes permit sequencing of significantly more samples for each operate without having compromising data good quality.
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Our instrument general performance assistance will help minimize unplanned downtime and reduce instrument requalification
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When utilizing LRM, i5 sequences needs to be entered into these software program while in the ahead orientation in all situations and for all instrument forms.
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For runs uploaded manually (i.e., an Illumina Instrument just isn't being used to make the sample sheet), the index orientation will depend on which instrument you are utilizing. Make sure you see the Index Orientation Desk for info on ways to enter the i5 sequence based on the instrument and software program utilized.
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Operates uploaded in guide method have their info immediately passed to bcl2fastq with no additional processing.
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The software program quickly generates the reverse enhance as necessary for analysis. In case the get more info sequence is reverse complemented, this may only be demonstrated within the SampleSheetUsed.
For your i5 index, evaluate color harmony using the forward orientation for MiSeq operates and MiniSeq runs with quick reagents, and the reverse-enhance orientation for all other devices.
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Go ahead and take guesswork out within your following workflow. Get recommendations and sources to help you sequence with assurance.